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Tomography and reconstituted slice images of internal structure of Mycoplasma mobile gliding machinery analyzed by negative staining electron tomography

Version 2 2024-06-03, 00:48
Version 1 2024-05-27, 07:15
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posted on 2024-06-03, 00:48 authored by Minoru Fukushima, Takuma Toyonaga, Yuhei Tahara, Daisuke Nakane, Makoto Miyata

  

Mycoplasma mobile, a parasitic bacterium glides by a special mechanism. We isolated and analyzed the gliding machinery by electron tomography. Here, we share tomography and reconstructed structures, as explained below.


Movie 1. Electron Tomography of isolated internal structures. Single images every 3° tilt series from −60° to +60° are assembled. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Figs. 2A, 2D, 2E, 2F, and 2G in the below RELATED MATERIAL.


Movie 2. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 75.2 nm high are stacked for 50 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Fig. 2A and 2D–2G in the below RELATED MATERIAL.


Movie 3. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 76.7 nm high are stacked for 51 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Figs. 2B in the below RELATED MATERIAL.


Movie 4. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 10.8 nm high are stacked for 67 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Figs. 2C in the below RELATED MATERIAL.


Movie 5. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 81.2 nm high are stacked for 54 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Figs. 3A–3F in the below RELATED MATERIAL.


Movie 6. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 67.7 nm high are stacked for 45 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Fig. 3G in the below RELATED MATERIAL.


Movie 7. Slice movie of chemically fixed and isolated internal structure reconstructed from a tilt series. Slice images 153.4 nm high are stacked for 102 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Figs. 4A–4F in the below RELATED MATERIAL.


Movie 8. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 90.2 nm high are stacked for 60 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Figs. 4G–4I in the below RELATED MATERIAL.

Funding

Three types of motility mechanisms in pathogenic bacteria, class Mollicutes

Japan Society for the Promotion of Science

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Construction of cell evolution model using synthetic bacterium JCVI syn3.0B and genome manipulation

Japan Science and Technology Agency

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History

Corresponding author email address

miyata@omu.ac.jp

Title (in Japanese)

ネガティブ染色電子線トモグラフィーで解析したマイコプラズマ・モービレ滑走装置内部構造のトモグラフィーと再構築されたスライス画像

Manuscript title (in Japanese)

ネガティブ染色電子線トモグラフィーで解析したマイコプラズマ・モービレ滑走装置の内部構造

Authors (in Japanese)

福島秀実,豊永拓真,田原悠平,中根大介,宮田真人

Copyright

© 2024 Author(s)

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