Tomography and reconstituted slice images of internal structure of Mycoplasma mobile gliding machinery analyzed by negative staining electron tomography

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posted on 2024-06-03, 00:48 authored by Minoru Fukushima, Takuma Toyonaga, Yuhei Tahara, Daisuke Nakane, Makoto Miyata

Mycoplasma mobile, a parasitic bacterium glides by a special mechanism. We isolated and analyzed the gliding machinery by electron tomography. Here, we share tomography and reconstructed structures, as explained below. Movie 1. Electron Tomography of isolated internal structures. Single images every 3° tilt series from −60° to +60° are assembled. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Figs. 2A, 2D, 2E, 2F, and 2G in the below RELATED MATERIAL. Movie 2. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 75.2 nm high are stacked for 50 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Fig. 2A and 2D–2G in the below RELATED MATERIAL. Movie 3. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 76.7 nm high are stacked for 51 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Figs. 2B in the below RELATED MATERIAL. Movie 4. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 10.8 nm high are stacked for 67 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Visualization of Isolated Gliding Machinery” and Figs. 2C in the below RELATED MATERIAL. Movie 5. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 81.2 nm high are stacked for 54 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Figs. 3A–3F in the below RELATED MATERIAL. Movie 6. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 67.7 nm high are stacked for 45 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Fig. 3G in the below RELATED MATERIAL. Movie 7. Slice movie of chemically fixed and isolated internal structure reconstructed from a tilt series. Slice images 153.4 nm high are stacked for 102 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Figs. 4A–4F in the below RELATED MATERIAL. Movie 8. Slice movie of isolated internal structure reconstructed from a tilt series. Slice images 90.2 nm high are stacked for 60 slices. Horizontal edge of movie is 1540 nm. The movie corresponds to the Results section: “Bell Structure” and Figs. 4G–4I in the below RELATED MATERIAL.