Observation of protoplasmic streaming over the entire body of Physarum plasmodium in the development of vein network.

<p><strong>Movie file “1/3_Exp1_Overview_2020.Nov.3”.</strong> </p> <p>Timelapse movie of development of vein-network in Physarum plasmodium . Time was indicated at the upper left corner, counted from 0hr 0min 50sec to 2hr 24min 50sec.  At 0hr 0min 50sec, The size of the organism observed was approximately 1 mm at the beginning of the observation. The flow of intracellular particles was observed to change while the vein network developed with rhythmic deformation of the organism. However, the flow of particles was difficult to recognize. This movie corresponds to the below RELATED MATERIAL: the subsection “Development of network of flow channels in the plasmodial droplet” in the section of Results of biological experiment and Figs. 4A, 4B, 4C, 4D.</p> <p><br></p> <p><strong>Movie file “2/3_Exp1_Raw_2020.Nov.3_1h_45m-47m”.</strong> </p> <p>Movie of protoplasmic streaming before the image processing for detecting the intrinsic intracellular moving particles in Physarum plasmodium (Exp 1). Time was indicated at the upper left corner, counted from 1hr 45min 00sec to 1hr 46min 59sec. This movie corresponds to the below RELATED MATERIAL: the subsection “Development of network of flow channels in the plasmodial droplet” in the section of Results of biological experiment and Fig. 4C.</p> <p><br></p> <p><strong>Movie file “3/3_Exp1_Visualized_particles_2020.Nov.3_1h_45m-47m”.</strong> </p> <p>Movie of protoplasmic streaming after the image processing for detecting the intrinsic intracellular moving particles in Physarum plasmodium (Exp 1). To visualize the flow of particles, a method of image analysis called Particle Tracking Velocimetry was used.  Moving particles were indicated as highly contrasted black dots. Time was indicated at the upper left corner, counted from 1hr 45min 00sec to 1hr 46min 59sec.This movie corresponds to the below RELATED MATERIAL: the subsection “Development of network of flow channels in the plasmodial droplet” in the section of Results of biological experiment and Fig. 4C’, and the subsection “Image processing for detecting the intrinsic intracellular moving particles” in the Materials and methods section and Fig. 2D.</p>