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serial electron microscopic and fluorescent images

figure
posted on 2022-12-28, 04:03 authored by satoru takahashi, chieko saito, Ikuko Koyama-Honda, Noboru Mizushima

Serial electron microscopic and fluorescent images of MEFs

The electron microscopic images were observed by scanning electron microscopy using array tomography. The sections for serial electron microscopic images were cut with 25 nm thickness. The MEFs expressed EGFP-LC3 and LAMP1-Halotag and starved for 30 minutes of serum and amino acid.

Funding

Mizushima Intracellular Decomposition Dynamics Project

Japan Science and Technology Agency

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Elucidation of the function of lipid-modified ATG8 in autolysosomal formation

Japan Society for the Promotion of Science

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Organelle particle Intracellular organelle transition developed by multi-parameter measurement

Japan Society for the Promotion of Science

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Research on intracellular degradation focusing on membranous structures

Japan Society for the Promotion of Science

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History

Corresponding author email address

nmizu@m.u-tokyo.ac.jp

Copyright

©2022 The Author(s)