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serial electron microscopic and fluorescent images

figure

posted on 2022-12-28, 04:03 authored by satoru takahashi, chieko saito, Ikuko Koyama-Honda, Noboru Mizushima

Serial electron microscopic and fluorescent images of MEFs

The electron microscopic images were observed by scanning electron microscopy using array tomography. The sections for serial electron microscopic images were cut with 25 nm thickness. The MEFs expressed EGFP-LC3 and LAMP1-Halotag and starved for 30 minutes of serum and amino acid.