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Raw datasets acquired to investigate the possible roles of CAHS proteins from tardigrade in osmotic stress tolerance in mammalian cells and their analysis program

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posted on 2024-12-04, 07:52 authored by Takahiro Bino, Yuhei Goto, Gembu Maryu, Kazuharu ArakawaKazuharu Arakawa, Kazuhiro Aoki

The data set can be classified into four principal categories.

・The raw image data obtained by time-lapse imaging (TIF format).

・The numerical data of mEGFP fluorescence intensity of each cell, which was used to quantify expression levels (CSV format).

・The raw data of the WST-1 and LDH assays (txt format).

・The Python program code (ipynb format).


The names of the files indicate which figure in Related Materials 1 they correspond to. 


In order to utilize the Python program code, it is necessary to refer to the file whose name corresponds to the respective filename.

To illustrate, in order to utilize the "Fig2_C_PythonProgram.ipynb", it is necessary to refer to the "Fig2_C_CAHS1-mEGFP_dox-0ng.csv", "Fig2_C_CAHS1-mEGFP_dox-1ng.csv", "Fig2_C_CAHS1-mEGFP_dox-10ng.csv", and "Fig2_C_CAHS1-mEGFP_dox-100ng.csv" files. 

Please note that the file path can be changed.


Further details on the analytical methodology can be found in the Methods section of  Related Materials 1

Funding

Gating mechanism of ERK and AKT in cell cycle checkpoint

Japan Society for the Promotion of Science

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Japan Society for the Promotion of Science

Search and functional analysis of tardigrade-specific non-domain proteins

Japan Society for the Promotion of Science

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Takeda Science Foundation

NAGASE Science Technology Foundation

History

Corresponding author email address

aoki.kazuhiro.6v@kyoto-u.ac.jp

Copyright

© 2024 The Author(s)

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