Collection times and locations of mosquito species collected by light traps or suction tubes in the evening and at night at various locations on the Jahangirnagar University campus in Dhaka, Bangladesh from 2015 to 2016.

Sampling methods.  We checked mosquito density in six rooms in the student hall. A bed net was hung in a room with 1 male volunteer inside, and mosquito collections were made outside of the bed net. One person collected mosquitoes from each room for 5 minutes in six rooms within one hour. Two persons rotated between rooms, so that two persons collected 10 minutes-person per room within one hour. This collection started at 5:00 pm and continued until 10:00 pm for five cycles, with each room being sampled with an aspirator for 50 minutes over a five-hour period. In the hall, we repeated the samplings in three seasons, pre-monsoon (2015, March 9, 10, 2015), monsoon (2015, August 13, 14) and winter (2016, February 18, 19) . All sampled rooms located with more than 20-meter intervals. We did the relevant samplings at four cattle houses in Kalabagan village on June 9 and 10 in 2015 and in the hat on August 14 and 15 and September 4 and 5, 2015. In the cattle houses, we collected mosquitoes using light traps and aspirators. We were concerned that light trap collection in a bright room might be ineffective, so we conducted aspiration sampling in the student hall. On the other hand, light trap collection is often applied to collect JE vector mosquitoes in animal houses. As a reference of sampling efficacy, we conducted both light trap collection and hourly aspiration (17PM to 22PM) in the four cowsheds in the village. Two CDC light traps with a 4-wat bulb hung one meter above the ground in the cattle sheds at 17pm and collect the mosquito next morning, at 8am. In the hat we collected mosquitoes by 10 minutes hourly aspiration from outside of bed nets with one volunteer inside during 17 to 22PM. Collected mosquitoes were transported to the laboratory and killed with chloroform. All mosquitoes were identified species using morphological keys.