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Primer sets used for quantitative real-time PCR of differentially expressed genes (DEGs) identified from RNA-Seq between normal and stem browning samples of the mushroom Lentiula edodes

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posted on 2024-10-04, 02:10 authored by Jili Zhang, Yuki Tanaka, Akiko Ono, Takumi Sato, Toshiyuki Suzuki, Saya Akimoto, Yuki Tanaka, Shoko Iwami, Aya Iwamoto, Norio Tanaka, Naotake Konno, Tomohiro Suzuki
Based on the results of the RNA-Seq analysis of black and normal stems in Lentinula edodes, quantitative reverse-transcription PCR (qRT-PCR) was performed to confirm the expression levels of 17 differentially expressed genes (DEGs) annotated as transcription factors and oxidoreductases. The primer sets used during this process are shown in this Table.

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Corresponding author email address

suzukit@s.utsunomiya-u.ac.jp

Title (in Japanese)

Lentiula edodesの黒柄と正常柄のRNA-Seqの解析結果をもとに17遺伝子を選抜した後に、リアルタイムPCRによる発現定量を行う際に使用したプライマー

Description (in Japanese)

Lentiula edodesの黒柄および正常柄のRNA-Seq解析の結果、17種類の転写因子および酸化還元酵素としてアノテーションされた発現差異遺伝子(DEGs)の発現レベルを確認するために、qRT-PCRを行った。その際に使用されたプライマーセット示した。

Authors (in Japanese)

張 吉麗、田中 祐基、小野 晶子、佐藤 匠、鈴木 稔之、秋本 紗弥、田中 祐生、岩見祥子、岩本 綾、田中 徳夫、金野 尚武、鈴木 智大

Copyright

© 2024 Jili Zhang, Yuki Tanaka, Akiko Ono, Takumi Sato, Toshiyuki Suzuki, Saya Akimoto, Yuki Tanaka, Shoko Iwami, Aya Iwamoto, Norio Tanaka, Naotake Konno, Tomohiro Suzuki

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